Both integration sequences were placed partially on top of the coding sequence, so that the deletion caused by the knock-out cassette integration would be only 215bp. Because Ku70 sequesters Bax from mitochondria, cells overexpressing Bax alone are more susceptible to apoptosis than are cells overexpressing both Bax and Ku70. In the presence or absence of Ku70 overexpression, 293T cells grown in serum from CR animals were less susceptible to Bax-mediated apoptosis as compared to cells grown in media with AL serum ( Fig. 1D ). The Ku70 NLS contains five lysines (K539, K542, K544, K553 and K556) that were identified as targets for acetylation in vivo. 18, 29 Lysine acetylation in the cytoplasm drives and coordinates key events such as cytoskeleton dynamics, intracellular trafficking, vesicle fusion, metabolism and stress response. 43, 44 We hypothesized that acetylation of the five lysine residues in the Ku70 NLS 2001-03-01 · Ku70:Ku80 complex Source: GO_Central "Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium." Gaudet P. , Livstone M.S. , Lewis S.E. , Thomas P.D. Brief Bioinform 12:449-462(2011) [ PubMed ] [ Europe PMC ] [ Abstract ] Anti-Ku70 Antibody (E-5) is a mouse monoclonal IgG 1 κ Ku70 antibody, cited in 60 publications, provided at 200 µg/ml; raised against amino acids 302-609 mapping at the C-terminus of Ku70 of the Ku protein of human origin We present evidence that inactivation of the Ku70 gene leads to a propensity for malignant transformation both in vitro and in vivo.

Ku70

Immunogen Synthetic peptide corresponding to Human Ku70 aa 550 to the C-terminus conjugated to keyhole limpet haemocyanin. (Peptide available as ab92421) Size: 609 amino acids Molecular mass: 69843 Da Quaternary structure: Heterodimer composed of XRCC5/Ku80 and XRCC6/Ku70. The dimer associates in a DNA-dependent manner with PRKDC to form the DNA-dependent protein kinase complex DNA-PK, and with the LIG4-XRCC4 complex to form the core of the non-homologous end joining (NHEJ) complex. Ku70 is required for late B cell development and immunoglobulin heavy chain class switching Immunoglobulin (Ig) heavy chain (HC) class switch recombination (CSR) is a late B cell process that involves intrachromosomal DNA rearrangement.

Rabbit anti Ku70 antibody recognizes human Ku70, also known as XRCC6, G22P1 or X-ray repair cross-complementing protein 6.

The Ku heterodimer binds nonspecifically to DNA ends with high affinity (K d: 1–2 nM) , and FOXO4 contains a DNA binding domain required for binding Forkhead sites on DNA . 2012-06-29 · The KU70 flipper knock-out cassette was constructed with 1299bp 5′ and 1170bp 3′ flanking sequences from the KU70 locus.

This plasmid is available through Addgene. Se hela listan på academic.oup.com of the Ku70/80 heterodimer in vitro. We further deﬁned the function of the Ku70 and Ku80 C-terminal domains in DNA end binding and DNA-PKCS interaction. 2. Results 2.1.

Ku70 is involved in repair of double stranded DNA breaks via the non-homologous end joining (NHEJ) pathway. Ku70 forms a heterodimer with Ku80 (Ku70/Ku80), which binds to the ends of broken DNA where it recruits proteins for end-processing and ligation. Ku70 + Ku80 (2) Ku70+Ku80+DNA Ligase IV/LIG4+Histone H2A.X+gamma H2A.X+Double strand break repair – Non Homologous E (1) Applications Clear 2007-05-25 Ku70 acetylation and enhances DNA repair activity, suggest-ing that Ku70 acetylation may have an inhibitory role in DNA repair.12 Several studies, however, have demonstrated that the level of Ku70 correlates with radiosensitivity.13 Ku70 knock down increases … KU70 – Kontrolluppgift – Näringsbidrag och royalty (SKV 2316) På skatteverket.se använder vi kakor (cookies) för att webbplatsen ska fungera på ett bra sätt för dig. Genom att surfa vidare godkänner du att vi använder kakor.
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Fig. 1. Spermatogenesis in Ku70 and Ku70 Rap1 knockout mice. (A) The testes of Ku70 2 /and Ku70 2/ Rap1 D mice are of reduced size, as shown for wild-type (left) and Ku70 2/ (right) testes. Scale bar: 5 mm. (B) Ku70 2/ and Ku70 2/ Rap1 D/ (dko) mice display a significant (P,0.001) reduction in testis size compared with wild type (WT) and The DNA end-joining protein Ku70 is one of several proteins that inhibit apoptosis by sequestering the proapoptotic factor Bax from the mitochondria.

18, 29 Lysine acetylation in the cytoplasm drives and coordinates key events such as cytoskeleton dynamics, intracellular trafficking, vesicle fusion, metabolism and stress response. 43, 44 We hypothesized that acetylation of the five lysine residues in the Ku70 NLS 2001-03-01 · Ku70:Ku80 complex Source: GO_Central "Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium." Gaudet P. , Livstone M.S. , Lewis S.E. , Thomas P.D. Brief Bioinform 12:449-462(2011) [ PubMed ] [ Europe PMC ] [ Abstract ] Anti-Ku70 Antibody (E-5) is a mouse monoclonal IgG 1 κ Ku70 antibody, cited in 60 publications, provided at 200 µg/ml; raised against amino acids 302-609 mapping at the C-terminus of Ku70 of the Ku protein of human origin We present evidence that inactivation of the Ku70 gene leads to a propensity for malignant transformation both in vitro and in vivo.
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Ku70 (also known as XRCC6) is a eukaryotic protein that is involved in the repair of DNA double-strand breaks by non-homologous end-joining [ 1, 2 ] Ku is a heterodimer of approximately 70kDa and 80kDa subunits The Ku70/Ku80 heterodimer has ATP-dependent DNA helicase activity and functions as the DNA-binding regulatory component of DNA-dependent protein kinase (DNA-PK) (6-8).

Spermatogenesis in Ku70 and Ku70 Rap1 knockout mice. (A) The testes of Ku70 2 /and Ku70 2/ Rap1 D mice are of reduced size, as shown for wild-type (left) and Ku70 2/ (right) testes. Scale bar: 5 mm. (B) Ku70 2/ and Ku70 2/ Rap1 D/ (dko) mice display a significant (P,0.001) reduction in testis size compared with wild type (WT) and The DNA end-joining protein Ku70 is one of several proteins that inhibit apoptosis by sequestering the proapoptotic factor Bax from the mitochondria. However, the molecular mechanism underlying Ku70-dependent inhibition of Bax is not fully understood. Here, we show that the absence of Ku70 results in the accumulation of ubiquitylated Bax. Under normal growth conditions, Bax ubiquitylation The protein of 70-kDa molecular weight is a DNA-helicase involved in the DNA double-strand break repair system (Double-Strand Break DNA Repair), more precisely the nonhomologous end joining.

2012-06-29 · The KU70 flipper knock-out cassette was constructed with 1299bp 5′ and 1170bp 3′ flanking sequences from the KU70 locus. Both integration sequences were placed partially on top of the coding sequence, so that the deletion caused by the knock-out cassette integration would be only 215bp. Because Ku70 sequesters Bax from mitochondria, cells overexpressing Bax alone are more susceptible to apoptosis than are cells overexpressing both Bax and Ku70. In the presence or absence of Ku70 overexpression, 293T cells grown in serum from CR animals were less susceptible to Bax-mediated apoptosis as compared to cells grown in media with AL serum ( Fig. 1D ). The Ku70 NLS contains five lysines (K539, K542, K544, K553 and K556) that were identified as targets for acetylation in vivo.